Research Paper Volume 13, Issue 11 pp 15193—15213

Figure 4. TRDMT1 mediates GCs oxidative DNA damage repair. (A) The DNA damage of H₂O₂+KGN+PBS and H₂O₂+KGN+NAC transfected with GFP-NC, GFP-TRDMT1, Sh-RNA and Sh-TRDMT1 was measured via comet assay. Scale bar: 100μm. Comparison of the tail of DNA between the groups. (B) H₂O₂+KGN+PBS transfected with GFP-NC, GFP-TRDMT1, Sh-RNA and Sh-TRDMT1 were harvested at the indicated time points for γ-H₂AX staining. Positive cells in each cell and the frequency of cells with foci were counted. Scale bar: 10μm. (C) H₂O₂+KGN+NAC transfected with GFP-NC, GFP-TRDMT1, Sh-RNA and Sh-TRDMT1 were harvested at the indicated time points for γ-H₂AX staining. Positive cells in each group and the frequency of cells with foci were counted. Scale bar: 10μm.*P<0.05, **p<0.01, and ***p<0.001. Statistical significance was determined using two-tailed t-tests for two groups and ANOVA for multiple comparisons. All values are the means ± SD.