Research Paper Volume 13, Issue 10 pp 13405—13420

Figure 5. CXCR2 enhances OC cell proliferation. BrdU immunofluorescence assays were conducted to investigate the role of CXCR2 on OC cell proliferation. In brief, cells were plated at the same density and fixed, as previously reported. Antibody against the marker of cell proliferation, BrdU, was conjugated with PE (red), whereas cells nuclei were stained with DAPI (blue). Finally, both staining conditions were merged for better visualization of the studied phenomenon. (A) A2780 cells were either transfected with empty vector for control (NC), siRNA CXCR2 (10μM) or treated with the CXCR2 antagonist SB225002 (1ug/ml). (B) ACRP cells were either transfected with empty vector for control (NC), siRNA CXCR2 (10μM) or treated with the CXCR2 antagonist SB225002 (1ug/ml). (C) BrdU positive rate, indicating cell proliferation under each specified experimental condition. Note that cell proliferation decreased significantly both in the KD models and under treatment of cells with SB225002. Cell proliferation was remarkably lower in ACRP cells vs. A2780 cells in all events. Figure is of a representative experiment. Data was analyzed by one-way ANOVA. *p<0.05. N=3. Imagines were acquired in 10x magnification.