Research Paper Volume 13, Issue 10 pp 14289—14303
Autophagy deficiency downregulates O6methylguanine-DNA methyltransferase and increases chemosensitivity of liver cancer cells
Figure 2. Loss of autophagy decreases MGMT expression. (A, B) HepG2 cells were infected with sh-Atg5/Atg7 HepG2 or empty lentivirus vector, treated with 2 μM epirubicin for 24 h, and levels of MGMT were determined by western blot assay; GAPDH was used as a loading control. (C) Western analysis of MGMT in wild-type HepG2 cells treated with 2 μM epirubicin for 24 h, followed by 24 h incubation with CQ. (D, E) Western analysis of MGMT in wild-type Huh7 cells infected with Atg5-shRNA lentivirus or Atg7-shRNA lentivirus, and treated with 2 μM epirubicin for 24 h. (F) Western analysis of MGMT in wild-type Huh7 cells treated 24 h with 2 μM epirubicin, followed by 24 h incubation with the indicated CQ concentrations.