Research Paper Volume 13, Issue 12 pp 15964—15989

Figure 6. Induction of autophagy in SW620 cells by AS treatment. (A) AS increases the ratio of Beclin-1/Bcl-2. Dose-dependent AS (100, 150, and 200 μg/mL) effects of AS on changes in Beclin-1 and Bcl-2 proteins were determined by Western blotting. (B) Relative changes in the ratio of Beclin1/Bcl-2 in accord with the dose were measured by commercially available quantitative software with the control representing 1-fold. Enhancement of AS-induced cell death by 3-MA and CQ inhibitors in SW620 cells. Cells were first treated with autophagy inhibitors (C) 3-MA (1.5 mM) and (D) CQ (10 μM) for 1 h and then incubated in presence or absence of AS (0-200 μg/mL) for 24 h. Cell viability was analyzed by the MTT assay. Results are expressed as the mean ± SD of three independent assays. ^*p < 0.05 is significant when compared with untreated control cells and ^#p < 0.05; ^##p < 0.01; ^###p < 0.001 is significant when compared with AS-treated cells.