Research Paper Volume 13, Issue 12 pp 15964—15989

Figure 3. AS induces mitochondrial dysfunction and Bax/Bcl-2 dysregulation in SW620 cells. (A, B) Depolarization of mitochondrial membrane potential (ΔΨm) was measured using Mito-tracker green detection kit. 100, 150, and 200 μg/mL of AS was used to treat cells for 24 h. AS increases the ratio of Bax/Bcl-2. (C) Dose-dependent (100, 150, and 200 μg/mL) effects of AS on Bax and Bcl-2 proteins were estimated through Western blotting. (D) Relative changes in the ratio of Bax and Bcl-2 with the different doses of AS were estimated by commercially available quantitative software with control as 1-fold. Values are expressed as the mean ± SD (n = 3). Statistical significance was defined as ^**p < 0.01 or ^***p < 0.001 when compared with control.