Research Paper Volume 13, Issue 16 pp 20094—20115

Figure 7. Detection of cell migration ability, and interaction between CDK1 and DEPDC1B. (A) The results of wound-healing assay and Transwell assay showed that, compared to NC(OE+KD) group: the migration rate of cells was significantly increased in DEPDC1B+NC(KD) group (48 h) (P<0.001), while in shCDK1+NC(OE) group (48 h), the migration rate of cells was significantly decreased (P<0.001). Compared to DEPDC1B+NC(KD) group, the migration rate of cells was significantly decreased in DEPDC1B+shCDK1 group (48 h) (P<0.001). Compared with shCDK1+NC(OE) group, the migration rate of cells was significantly increased in DEPDC1B+shCDK1 group (48 h) (P<0.05). (B) The transwell assay shows that, compared to NC(OE+KD) group: the migration ability of cells in DEPDC1B+NC(KD) group was significantly increased (P<0.001), and the migration ability of cells in shCDK1+NC(OE) group is significantly decreased (P<0.001). Compared to DEPDC1B+NC(KD) group, the migration ability of cells was significantly decreased in DEPDC1B+shCDK1 group (P<0.001). Compared with shCDK1+NC(OE) group, the migration ability of cells in DEPDC1B+shCDK1 group was significantly increased (P<0.001). (C, D) The results of co-immunoprecipitation. *: P <0.05. **: P <0.01. ***: P <0.001.