Research Paper Volume 13, Issue 16 pp 20094—20115

Figure 6. Functions on cell progression. (A) The results of Celigo cell counting assay show that, compared with NC(OE+KD) group: the cells in DEPDC1B+NC (KD) group exhibited faster proliferation rate (P<0.001), and the cells in shCDK1+NC(OE) group exhibited slower proliferation rate (P<0.001). Compared to DEPDC1B+NC(KD) group, the cells in DEPDC1B+shCDK1 group exhibited slower proliferation rate (P<0.01). The cells in DEPDC1B+shCDK1 group exhibit faster proliferation rate, compared with shCDK1+NC(OE) group (P<0.01). (B) The results of colony formation assay show that, compared to NC(OE+KD) group: the cell colony number in DEPDC1B+NC(KD) group was significantly increased (P<0.001), while the cell colony number in shCDK1+NC(OE) group was significantly decreased (P<0.001). Compared with DEPDC1B+NC(KD) group, cell colony number was significantly decreased in DEPDC1B+shCDK1 group (P<0.001). Cell colony number in DEPDC1B+shCDK1 group was significantly increased, compared to shCDK1+NC(OE) group (P<0.01). (C) The results of flow cytometry demonstrate that: compared to NC(OE+KD) group: cell apoptosis was decreased in DEPDC1B+NC(KD) group (P<0.01), and in shCDK1+NC(OE) group, cell apoptosis was significantly increased (P<0.001). Compared with DEPDC1B+NC(KD) group, cell apoptosis was significantly increased in DEPDC1B+shCDK1 group (P<0.001). Compared to shCDK1+NC(OE) group, cell apoptosis was decreased in DEPDC1B+shCDK1 group (P<0.001). **: P <0.01. ***: P <0.001.