Research Paper Volume 13, Issue 9 pp 13179—13194

Figure 4. Knockdown of C/EBPα attenuates ATRA-induced upregulation of NEAT1 and NB4 cell differentiation. (A) NB4 cells were transfected with 3 μg siRNA targeting C/EBPα (siC/EBPα) or negative control siRNA (NC). Six hours later, cells were treated with 1μM ATRA for 24 h. Expression of C/EBPα was subsequently determined by qRT-PCR. (B, C) Expression of NEAT1 and NEAT1_2 isoform in C/EBPα-silenced NB4 cells was detected both before and after ATRA treatment. (D, E) The granulocytic differentiation marker CD11b, CD18, and CD11c in C/EBPα-silenced NB4 cells were tested after ATRA treatment for 24 h. The data represent the mean ± S.E.M from three replicates. * indicates p<0.05. (F, G) The expression of NEAT1 and NEAT1_2 isoform in C/EBPα-silenced primary APL bone marrow cells was measured after ATRA treatment for 24 h.