Figure 5.Effect of HBOT on apoptosis using TUNEL staining in cardiomyocytes of pre-diabetic rats after induction of aging by D-gal. (A) Representative figure of TUNEL positive cells in sham-treated rats. (B) Representative figure of TUNEL positive cells in HBOT-treated rats. (C) Percentage of apoptotic index. NDV, normal diet fed rats with vehicle; NDDg, normal diet fed rats with D-gal; HFV, high-fat diet fed rats with vehicle; HFDg, high-fat diet fed rats with D-gal; TUNEL, Terminal deoxynucleotidyl transferase dUTP nick end labeling; HBOT, hyperbaric oxygen therapy. (n = 3/group). *P < 0.05, **P < 0.01 compared to other groups.
