Research Paper Volume 13, Issue 9 pp 12955—12972

Figure 6. Mitophagy is activated through the PINK1/Parkin pathway. (A, B) MDA-MB-231 cells were treated with the indicated concentrations of warangalone for 12 h. The mRNA expressions of PINK1 and Parkin were measured by PCR (A), and protein expressions by Western blotting (B). GAPDH was used as the loading control. (C) MDA-MB-231 cells were treated with 20 μM warangalone for 12 h, and then immunostained with PINK1 and Parkin antibody and stained with DAPI. A CLSM was used to observe the fluorescence expression. Parkin labeled with Alexa Fluor 594 emits red, PINK1 labeled with Alexa Fluor 488 emits green, and nuclei labeled with DAPI emit blue. One-way ANOVA was used for statistical analysis (n ≥ 3). *P < 0.05, **P < 0.01, ***P < 0.001 compared to the respective control group.