Research Paper Volume 13, Issue 9 pp 12817—12832

Figure 6. AMPK/ACC/CPT1 pathway was involved in membrane aging processes. Membrane aging activated protein expression of AMPK-α2 through Ser485/491 residue phosphorylation by DHA treatment. However, AMPK-α1 showed no significant change in the protein expression level (A, B). Membrane aging also increased AMPK-α2 mRNA levels (P < 0.01), and DHA co-treatment partly decreased mRNA levels of AMPK-α2 (P < 0.01) (C). AMPK-α1 mRNA levels decreased in the membrane aging group and slightly increased in the DHA treatment group (D). CPT1c protein and mRNA levels were also inhibited by membrane aging (P < 0.01) and eased by DHA intervention (P < 0.01) (B, E). The protein and mRNA expression of two downstream factors of AMPK were detected. Membrane aging inhibited the expression of ACC1 protein; DHA co-treatment comparatively increased the protein level (F). The ACC1 mRNA level followed the same trend, which was reduced by membrane aging (P < 0.05) and then ameliorated by DHA administration (P < 0.01) (G). All the data are expressed as mean ± SD from three independent experiments (N = 3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. One-way ANOVA was used to determine the statistical significance of the differences.