Research Paper Volume 13, Issue 9 pp 12479—12492

Figure 3. Inhibitory effects of SUMO1P3 knockdown on HCC cell proliferation in vitro. MHCC97H and HepG2 cells were not transfected (control) or transfected with siSUMO1P3 or siNC. (A) The expression of SUMO1P3 was detected by qPCR assays. GAPDH was used as the endogenous control. (B, C) Cell viability was measured using CCK8 assay at 1, 2, 3, and 4 days after transfection. (D) EdU staining was used to detect cell proliferation. Scale bar: 5 μm. (E) Percentage of EdU-positive staining in (D). (F) Representative photos of colony formation. (G) Colony number was calculated in (F). (H) Western blot was conducted to analyze the expression of cyclin D1, p-Akt, and Akt. β-actin was used as endogenous control. All data are represented as the mean ± SD of three replicates. *P < 0.05, **P < 0.01 vs. Ctrl or siNC group.