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Online ISSN: 1945-4589

Research Paper|Volume 13, Issue 8|pp. 12031–12045

Low magnitude vibration alleviates age-related bone loss by inhibiting cell senescence of osteogenic cells in naturally senescent rats

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Figure 3.Senescence identification of osteoblasts in aged and young rats. (A) 53BP1 immunofluorescence staining of aged rat and young rat osteoblasts; (B) H3K9Me3 immunofluorescence staining of aged rat and young rat osteoblasts; (C) Senescence marker protein assay and protein relative expression statistics of aged rat and young rat osteoblasts $(\overline{x}\pm s,\text{n}=3)$; (D) β-Galactosidase staining plots and statistical plots of the percentage of positive β-galactosidase staining cells $(\overline{x}\pm s,\text{n}=3)$; *P < 0.05 vs. young female rats, **P < 0.01 vs. young female rats, ^#P < 0.05 vs. young male rats, ^##P < 0.01 vs. young male rats.