Research Paper Volume 13, Issue 8 pp 12007—12015

Figure 4. LINC00514 sponged miR-204-3p/KRAS expression in GC cells. (A) LINC00514 may be a sponge gene for miR-204-3p by utilizing the bioinformatics tools in starBase. (B) It was noted that KRAS was a potential target gene for miR-204-3p according to TargetScan bioinformatic analysis. (C) The level of miR-204-3p was upregulated in HGC-27 cells after transfection with the miR-204-3p mimic. (D) Overexpression of miR-204-3p decreased the luciferase value of the LINC00514-wild-type reporter but not that under the mutant reporter. (E) Elevated expression of miR-204-3p suppressed the luciferase value of the KRAS-wild-type reporter but not that under the mutant reporter. (F) Elevated expression of miR-204-3p suppressed KRAS expression in HGC-27 cells. (G) Ectopic expression of LINC00514 inhibited miR-204-3p expression in HGC-27 cells. (H) Overexpression of LINC00514 promoted KRAS expression in HGC-27 cells. **p<0.01 and ***p<0.001.