Research Paper Volume 13, Issue 8 pp 11942—11953
Activation of GPR30 with G1 inhibits oscillatory shear stress-induced adhesion of THP-1 monocytes to HAECs by increasing KLF2
Figure 8. The effects of G1 on KLF2 expression are mediated by ERK5. (A) HAECs were exposed to OSS (5 dyn/cm2) in the presence or absence of 5, 10 μM G1 for 2 h. Phosphorylated levels were measured by western blot analysis; (B, C). HAECs were exposed to OSS (5 dyn/cm2) in the presence or absence of 10 μM G1 or the specific ERK5 inhibitor XMD8-92 (10 nM) for 24 h. The expression of KLF2 at the mRNA level and protein level were determined by real time PCR and western blot analysis, respectively (*, #, $, P<0.01 vs. previous column group, N=5).