Research Paper Volume 13, Issue 8 pp 11542—11563

Figure 5. HucMSC-ex promote SIRT1 expression level under oxidative stress and activates autophagy to alleviate HaCaT cells damage. (A) Western blot analysis of SIRT1 expression level in HaCaT cells treated with different intensity of UVB. (B) Western blot analysis of SIRT1 expression level in HaCaT cells treated with different concentrations of H₂O₂. (C) Western blot detection of SIRT1 expression level in HaCaT cells treated with H₂O₂ at different times. (D) Western blot detection of SIRT1 expression level after H₂O₂ treatment of HaCaT cells at different times. (E) Immunofluorescence detection of SIRT1 expression level after H₂O₂ treatment of HaCaT cells. (F) qRT-PCR was used to detect the expression level of SIRT1 and Nrf2 mRNA of HaCaT cells after 24hours treatment with H₂O₂ (n = 3; *p < 0.05, **p < 0.01, ***p < 0.001). (G) Western blot detection of PCNA, Bax, LC3II, LC3I and SIRT1 protein expression level. (H) Immunofluorescence detection of hucMSC-ex effect on autophagy associated protein LC3B expression level.