Research Paper Volume 13, Issue 8 pp 11542—11563

Figure 4. HucMSC-ex protect skin keratinocytes from oxidative stress. (A) The ROS production of HaCaT cells after 600μM H₂O₂ treatment was detected by the inverted fluorescence microscope. (B) Immunohistochemical staining of 8-OHDG expression level in HaCaT cells treated with 600μM H₂O₂. (C) Immunofluorescence detection of PCNA and Caspase3 expression level in HaCaT cells treated with 600μM H₂O₂. (D) qRT-PCR detection of the expression level of HaCaT cells inflammatory cytokines TNF-α (n = 3; *p < 0.05, **p < 0.01, ***p < 0.001).