Research Paper Volume 13, Issue 6 pp 7975—7997
Development and validation of a RNA binding protein-associated prognostic model for head and neck squamous cell carcinoma
Figure 10. Immunohistochemistry (IHC), mutations and verification results in the prognosis-related RBPs. The protein levels of (A) CELF2, (B) EZH2 and (C) SIDT1 were determined by immunohistochemistry using indicated antibodies in HPA database, the staining strengths were annotated as Not detected, Low, Medium and High. The bar plots indicating the number of samples with different staining strength in HPA database. (D) OncoPrint showing the copy number alterations and mRNA expression alterations of 9 RBPs in prognostic model. (E) The mRNA expression levels of CELF2, EZH2 and SIDT1 in HNSCC cell lines were determined by qRT-PCR, GAPDH was verified as a housekeeping gene. (F) Western blot was conducted to evaluate the protein expression of EZH2 in HNSCC cell. (G, H) Colony-forming assay was conducted to evaluate the growth of HNSCC cell lines.