Research Paper Volume 13, Issue 7 pp 10672—10687

Figure 4. NET1 knockdown suppresses HCC tumorigenesis, and inhibits intrahepatic and lung metastases in vivo. (A) Left panel: representative images of tumors generated by control and NET1-knocked down YY-8103 cells. Middle panel: tumor weight (g). Right panel: tumor growth (mm³). (B) Left panel: representative images showing luciferase expression in intrahepatic tumors of mice from YY-8103 control and shNET1 groups. Right panel: quantification of luciferase expression in intrahepatic tumors. (C) Survival curves of mice in YY-8103 control and shNET1 groups. (D) Representative images of mice livers from YY-8103 control and shNET1 groups with intrahepatic injection. (E) Foci number of injected (left image) and non-injected (right image) lobes of mice with intrahepatic metastasis. (F) Left image: representative images showing luciferase expression from metastasized lungs of mice from YY-8103 control and shNET1 groups. Right image: quantification of luciferase expression in metastasized lungs. All data are presented as mean ± SE. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs SCR cells.