Research Paper Volume 13, Issue 8 pp 11470—11490
Excessive expression of miR-1a by statin causes skeletal injury through targeting mitogen-activated protein kinase kinase kinase 1
Figure 5. The miR-1a-MAP3K1 pathway mediates statin-induced cell apoptosis in skeletal muscle cells. Cultured skeletal muscle cells were transfected with miR-1a inhibitor and/or MAP3K1 siRNA for 48 hours followed by statin treatment for 24 hours. (A–C) Cells were subjected to detect the protein levels of pro-/cleaved-caspase3 in (A) bcl-2 and bax in (B) and pro-/cleaved-caspase7, 9 in (C) by western blot. N is 5 in each group. **P<0.01 vs. statin alone. #P<0.05 or ##P<0.01 vs. statin plus miR-1a inhibitor. (D, E) Cell apoptosis was determined by TUNEL in (D) and flow cytometry in (E). *P < 0.05 or **P < 0.01 vs. statin plus miR-1a inhibitor. A one-way ANOVA followed by Tukey post-hoc tests was used to determine P value in (A–E).