Ischemic-hypoxic preconditioning enhances the mitochondrial function recovery of transplanted olfactory mucosa mesenchymal stem cells via miR-181a signaling in ischemic stroke

Yi Zhuo; Wei Chen; Wenshui Li; Yan Huang; Da Duan; Lite Ge; Jialin He; Jianyang Liu; Zhiping Hu; Ming Lu

doi:doi:10.18632/aging.202807

← Back

Research Paper Volume 13, Issue 8 pp 11234—11256

Ischemic-hypoxic preconditioning enhances the mitochondrial function recovery of transplanted olfactory mucosa mesenchymal stem cells via miR-181a signaling in ischemic stroke

Figure 4. Co-culture of IhOM-MSCs with SH-SY5Y neurons enhances the mitochondrial function recovery under OGD/R insult. (A, B) Mitochondrial membrane potential (JC-1-labeled) and ROS were evaluated by immunofluorescence. (C, D) The histogram of JC-1 and ROS immunofluorescence. (E) Mitochondrial membrane potential was measured for using the JC-1-labeled by flow cytometry. (F) Levels of ROS were evaluated by flow cytometry. (OM-MSCs were replaced by MSCs in the figure. All data are presented as the mean value ± SD. *p<0.05, **p<0.01).