Research Paper Volume 13, Issue 7 pp 10387—10395

MSC-AS1 induced cell growth and inflammatory mediators secretion through sponging miR-142-5p/DDX5 in gastric carcinoma


Figure 5. MSC-AS1 modulated miR-142-5p/DDX5 expression. (A) By utilizing an online tool, TargetScan, DDX5 was predicted to be a potential target gene of miR-142-5p. (B) The expression of DDX5 was measured by using qRT-PCR analysis. (C) Overexpression of miR-142-5p suppressed the luciferase activity of wild-type DDX5, but the luciferase activity of mutant DDX5 was not changed. (D) DDX5 and miR-142-5p were enriched in the Ago2-containing beads compared with the input, according to the RIP method. (E) miR-142-5p overexpression decreased DDX5 expression. (F) MSC-AS1 overexpression inhibited miR-142-5p expression. (G) The expression of DDX5 was measured by qRT-PCR assay. **p<0.01.