Research Paper Volume 13, Issue 8 pp 11061—11082
microRNA-363-3p reduces endothelial cell inflammatory responses in coronary heart disease via inactivation of the NOX4-dependent p38 MAPK axis
Figure 3. Reduced miR-363-3p expression and abundant NOX4 and p38 MAPK expressions in HCY-induced CAECs. (A) positive and negative staining for goat antibody to CD34 (scale bar = 25 μm); (B) positive and negative staining for rabbit antibody to Factor VIII (scale bar = 25 μm); (C) miR-363-3p expression and NOX4 mRNA expression were determined by RT-qPCR in HCY-induced CAECs; (D) Representative Western blots of NOX4, p38 MAPK and p-p38 MAPK proteins and their quantitation in HCY-induced CAECs, normalized by β-actin. The data were analyzed by paired t-test. n = 3. * p < 0.05 vs. the control group; CAECs, coronary arterial endothelial cells; SP, streptavidin-peroxidase.