Research Paper Volume 13, Issue 7 pp 9627—9645

Figure 7. Hsa_circ_0053063 activated PDCD4 through targeting miR-330-3p. (A–B) qRT-PCR analysis revealed the expression of PDCD4 after transfecting miR-330-3p mimics, hsa_circ_0053063 siRNA, and LV-circ-0053063 in MDA-MB-231 and MDA-MB-468 cells. (C–D) qRT-PCR analysis revealed the expression of PDCD4 mRNA in MDA-MB-231 and MDA-MB-468 cells treated with miR-330-3p mimics and inhibitors. (E–F) Western blot assays were employed to detect the protein levels of PDCD4 in MDA-MB-231 and MDA-MB-468 cell lines transfected with lv-circ_0053063. (G–H) Western blot assays were employed to detect the protein levels of PDCD4 in MDA-MB-231 and MDA-MB-468 cell lines transfected with miR-330-3p mimics. (^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001).