Research Paper Volume 13, Issue 7 pp 9566—9581

Figure 4. PTEN is a target of miR-20b, IL6R is a target of miR125a. (A) Analysis of the luciferase reporter assay. Firefly luciferase reporters containing either WT or MUT miR-20b binding sites in the PTEN 3’-UTR were co-transfected into KYSE-150 and KYSE-150R cells with miR-20b and miR-20b control. (B) Analysis of the luciferase reporter assay. Firefly luciferase reporters containing either WT or MUT miR-125a binding sites in the IL6R 3’-UTR were co-transfected into cells with miR-125a and miR-125a control. (C, D) Western blot (C) and RT-PCR (D) analysis of PTEN expression level in cells transfected with miR-20b mimic or inhibitor. (E, F) Western blot (E) and RT-PCR (F) analysis of IL6R expression level in cells transfected with miR-125a mimic or inhibitor. *P<0.05 by Student’s t-test. MUT, mutant; WT, wild type; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.