Research Paper Volume 13, Issue 6 pp 8665—8687
MicroRNA-95-3p serves as a contributor to cisplatin resistance in human gastric cancer cells by targeting EMP1/PI3K/AKT signaling
Figure 8. Down-regulation of EMP1 led to improved cell migration and invasion via activation of the EMT process and PI3K/AKT pathway. (A, B) Transwell chamber assay demonstrated that knockdown of EMP1 led to increased invasion and migration compared to the miR-95-3p inhibitor group. (C) Wound healing assay further verified that knockdown of EMP1 led to increased cell migration compared to the miR-95-3p inhibitor group. (D) Western blot assay indicated that knockdown of EMP1 induced up-regulation of neoplastic proteins (Bcl-xl and Bcl-2), activation of EMT and PI3K/AKT pathway compared to the miR-95-3p inhibitor group. ***p<0.001, **p<0.01.