Research Paper Volume 13, Issue 6 pp 8643—8664
Follistatin-like protein 1 functions as a potential target of gene therapy in proliferative diabetic retinopathy
Figure 8. The expression of FSTL1, TGFβ, CTGF, VEGF, FN, COL1 and α-SMA in HUVECs. In Immunofluorescence figures, (A) indicates normal cell fluorescence, hypoxic 3 h cell fluorescence and hypoxic 6 h cell fluorescence (10×). (B–H) indicates the quantitative analysis of immunofluorescence figures in the normal, 3 h hypoxia, and 6 h hypoxia models using ImageJ. The 3 h and 6 h hypoxia results indicated that FSTL1, TGFβ1, CTGF, VEGF, FN, COL1, and α-SMA were expressed at significantly higher levels than in normal cells. (I–O) indicates that the mRNA expression of FSTL1 and ECM-related factors in the three models. The expression of FSTL1, TGFβ1, CTGF, VEGF, FN, COL1, and α-SMA mRNA in 3 h and 6 h hypoxia conditions, respectively, were expressed at significantly higher levels than in normal cells (N: normal, h3: 3 h hypoxia, h6: 6 h hypoxia). Data are expressed as mean ± SEM. * means P < 0.05, **means P < 0.01, *** means P < 0.001.