Research Paper
miR-128 participates in the pathogenesis of slow transit constipation by regulating the p38α/M-CSF inflammatory signaling pathway
Figure 4. miR-128 interference in CT26.WT cells affect expression of miR-128 toward p38α/MAPK and the downstream genes AKT and M-CSF. (A, B) miR-128-2-5p, M-CSF, p38α and AKT mRNA expression levels in cells trans-infected with micrON mmu-miR-128-2-5p mimic/micrON mimic NC; (C, D) miR-128-2-5p, M-CSF, p38α and AKT mRNA expression levels in cells trans-infected with mmu-miR-128-2-5p inhibitor/ micrOFF inhibitor NC. (E) protein expression levels of M-CSF, p38α in CT26.WT cells trans-infected with micrON mmu-miR-128-2-5p mimic/micrON mimic NC/ micrOFF mmu-miR-128-2-5p inhibitor/ micrOFF inhibitor NC. *P<0.05, **P<0.01.