Research Paper Volume 13, Issue 8 pp 11026—11042

Figure 1. Effects of calycosin on the proliferation of ECs and BCCs. (A–C) HUVECs, HMEC-1 cells, MCF-7 cells and T47D cells were treated with calycosin (1-60 μM) or E₂ (10 nM) for 12, 24, or 48 h. Cell proliferation was determined using a CCK-8 assay. (D) HUVECs, HMEC-1 cells, MCF-7 cells and T47D cells were treated with calycosin (20 μM) or E₂ (10 nM) for 12, 24, or 48 h. Cell proliferation was determined using the BrdU assay. (E) For the colony formation assays, after treatment with calycosin (20 μM) or E₂ (10 nM) for 7, 14, and 21 days, the numbers of cell colonies were counted. The results are from three independent experiments performed in triplicate. *p < 0.05 vs. control (0 μM).