Figure 6. ENAM is down-regulated in ccRCC. (A) Expression of ENAM was down-regulated in 72 paired tumor compared with paratumor samples in TCGA; (B) Expression of ENAM was frequently down-regulated in 50 ccRCC tumor tissue; (C) ENAM protein expression in HK-2, OSRC-2, SW839, Caki-1 and A498 cell lines; (D) qPCR of indicated cells transfected with ENAM-vector and ENAM; (E) Western blotting of indicated cells transfected with ENAM-vector and ENAM. Abbreviations: NS: P>0.05; *: P<0.05; **: P<0.01; ***: P<0.001.