Research Paper Volume 13, Issue 4 pp 5986—6009

Figure 5. Knockdown of miR1273f could reduce PTX sensitivity in A549 cell line while overexpression of miR1273f could reverse PTX resistance in A549/Taxol cell line. (A) Cell proliferation ability of A549 and A549/Taxol cells treated with PTX after transfection of inhibitor-miR1273f and mimic-miR1273f was evaluated by colony formation assay and crystal violet staining assay. (B) The stained cell area ratio was calculated by 15 random fields per well in Figure 5A (images acquired at 10× magnification). (C) OD values of 4 groups with A549 cells were detected at 595 nm using the NanoDrop ND-1000 spectrophotometer. (D) OD values of 4 groups with A549/Taxol cells were detected at 595 nm using the NanoDrop ND-1000 spectrophotometer. (E) Cell viability of A549 and A549/Taxol cells treated with PTX after transfection of inhibitor-miR1273f and mimic-miR1273f was evaluated by CCK-8 assay. The absorbance at 450 nm of 4 groups was measured using NanoDrop ND-1000 spectrophotometer. *p <0.05, **p <0.01, ***p <0.001, n=3 in each group. Scale bar=10mm (upper) or 500μM (lower).