Research Paper Volume 13, Issue 4 pp 5986—6009

Figure 4. Knockdown or overexpression of miR1273f regulated the expression of MDM2 and P53 in A549 cell line. (A) miR1273f inhibitor specifically targeting the binding sites in miR1273f was designed. (B) miR1273f was determined by qPCR method after transfection of inhibitor-miR1273f and mimic-miR1273f. (C) MDM2 mRNA level was determined by qPCR method after transfection of inhibitor-miR1273f and mimic-miR1273f. (D) MDM2 and P53 expression were determined by western blot after transfection of inhibitor-miR1273f and mimic-miR1273f. (E) The quantification of MDM2 protein was analyzed according to bands in Figure 4D. (F) The quantification of P53 protein was analyzed according to bands in Figure 4D. *p <0.05, **p <0.01, ***p <0.001, n=3 in each group.