Research Paper Volume 13, Issue 4 pp 5858—5874

Figure 3. Low GABARAP level promotes cellular EMT via AKT/mTOR signaling in breast cancer. (A) Western blot analyses were used to detect the expression levels of E-cadherin, N-cadherin, vimentin, MMP2, MMP14, p-AKT, AKT, p-mTOR, mTOR, p-p70s6k and p70s6k in T47D-vector, T47D-shRNA, UACC-812-vector and UACC-812-shRNA cells. Cells were lysed using RIPA lysis buffer containing protease inhibitors and a phosphorylase inhibitor cocktail to obtain protein. β-actin was used as an internal control. (B) Western blot analyses were used to detect the expression levels of E-cadherin, N-cadherin, vimentin, MMP2, MMP14, p-AKT, AKT, p-mTOR, mTOR, p-p70s6k and p70s6k in MDA-MB-453-vector and MDA-MB-453-GABARAP cells. Cells were lysed using RIPA lysis buffer containing protease inhibitors and a phosphorylase inhibitor cocktail to obtain protein. β-actin was used as an internal control. (C) Pearson correlation was calculated among genes related to GABARAP, CDH1, MMP2, MMP14 and the AKT/ mTOR signaling pathway in breast cancer patients clinical cohort (TCGA).