Figure 8. MIF activated signaling pathways related to inflammation in JFs. (A–D) Western blot analysis of MAPK signaling-related proteins p-ERK (A), p-P38 (B), p-JNK(C), and NF-κB signaling-related protein p-NF-κB (D) after JFs were treated with 2 μg/mL recombinant MIF for 0, 15, 30, 60, and 120 min. Error bars represent standard deviation. *P <0.05 compared with the 0 min group.