COVID-19 Research Paper Volume 13, Issue 2 pp 1571—1590

Figure 3. Effects of novel C. sativa extracts on the levels of IL-6 and COX2 in human EpiDerm FT tissues. To induce inflammation, tissues were exposed to UV. Upon exposure, tissues were incubated with extracts or vehicle (DMSO) for 24 h. Three tissues were used for each condition. Western blot analysis was performed using antibodies against IL-6 and COX2 as detailed in the Methods section. “UNT” – untreated tissues; “PBS” - 15 μl of 30% glycerol in PBS was applied to the tissues and no exposure was done; “DMSO” - 15 μl of DMSO (0.017% in 30% glycerol-PBS) was applied to the tissues and no exposure was done; “UV-PBS” - tissues were exposed to UV and 15 μl of 30% glycerol-PBS was applied to them; “UV-DMSO” – tissues were exposed to UV and 15 μl of DMSO (0.017% in 30% glycerol-PBS) was applied to them; “#4” through “#15” - tissues were exposed to UV and 15 μl of extracts in DMSO was applied to them.