Figure 9. Effect of melatonin on the expression of zygote genome activation and pluripotency related genes in EB1-exposed embryos. (A–D) relative expression levels of Eifla, Oct4, Nanog and Sox2, respectively. CON, the control group. EB1, embryos treated with EB1. MT, embryos treated with melatonin. ME, embryos treated with both melatonin and EB1. a-dValues for a given group in columns with different superscripts differ significantly (p < 0.05).