Research Paper Volume 13, Issue 3 pp 4063—4078
PRPS1-mediated purine biosynthesis is critical for pluripotent stem cell survival and stemness
Figure 3. PRPS1/PRPS2 overexpression has no effect on PSCs stemness. (A, C) qRT-PCR analysis of expression levels of pluripotency genes, Oct4, Nanog, c-Myc, and Sox2, in PSCs (CB iPSCs) (A) and EB (embryoid bodies) (C). *P< 0.05. (B, D) qRT-PCR analysis of expression levels of triploblastic genes, ENG, FOXA2, NES, and NT5E, in PSCs (B) and EB (embryoid bodies) (D) Data are representative of three independent experiments with similar results.