Research Paper Volume 13, Issue 3 pp 3909—3925
Dysregulation of USP18/FTO/PYCR1 signaling network promotes bladder cancer development and progression
Figure 4. Turn down USP18 or FTO inhibits BLCA cell proliferation and migration. (A) Time-dependent BLCA cell proliferation upon depletion of USP18 by MTT assay. USP18 was knocked down by transfecting USP18 siRNA-248 (si-USP-1) and USP18 siRNA-581 (si-USP-2). ** p < 0.01, *** p < 0.001 vs. control, student’s t-test. (B) Wound-healing assay assessing the role of USP18 in BLCA cell migration. USP18 was knocked down by transfecting USP18 siRNA-248 (si-USP-1) and USP18 siRNA-581 (si-USP-2). (C) MTT assay assessing time-dependent BLCA cell proliferation upon depletion or expression of FTO, or depletion of USP18 together with FTO overexpression. *p < 0.05, **p < 0.01 vs. control, student’s t-test. (D) Wound-healing assay assessing BLCA cell migration upon depletion or expression of FTO, or depletion of USP18 together with FTO overexpression.