Research Paper Volume 13, Issue 1 pp 1357—1368
miR-18a increases insulin sensitivity by inhibiting PTEN
Figure 3. miR-18a enhances insulin-stimulated AKT phosphorylation/activation in insulin target cells. (A, B) MiR-18a levels were detected using qRT-PCR in 3T3-L1 and C2C12 cells in which miR-18a was overexpressed (LV-miR-18a) (A) or inhibited (anti-miR-18a) (B). (C, D) Western blot analysis of insulin-stimulated AKT phosphorylation in 3T3-L1 (C) and C2C12 (D) cells overexpressing miR-18a. (E, F) Western blot analysis of insulin-stimulated AKT phosphorylation in 3T3-L1 (E) and C2C12 (F) cells transfected with miR-18a inhibitors (mean ± SD, *P<0.05).