Research Paper Volume 13, Issue 1 pp 1001—1016

Figure 3. Wntless^Flox/Flox, Amhr2-Cre females displayed subfertility with normal oocyte and embryonic development. (A) Comparison of the accumulative number of pups per Wntless^Flox/Flox, Wntless^Flox/-, Ddx4-Cre, Wntless^Flox/Flox, Gdf9-Cre, and Wntless^Flox/Flox, Amhr2-Cre females (n = 6 for each group). (B) Histological ovarian images of Wntless^Flox/Flox and Wntless^Flox/Flox, Amhr2-Cre females. Scale bar = 100 μm. The ovaries were collected from 6-wk-old mice with a random cycle. (C) 345 and 400 GV oocytes from Wntless^Flox/Flox and Wntless^Flox/Flox, Amhr2-Cre females were matured in M16 medium, respectively. After maturing for 3 and 16 h, oocytes were counted, and the rates for GVBD and PB1 were calculated, respectively. Representative images of mouse oocytes at GVBD and PB1 stages are shown above the bar graph. (D) Zygotes collected from Wntless ^Flox/Flox (n=160) and Wntless^Flox/Flox, Amhr2-Cre (n=193) females with an obvious vaginal plug after mating with wildtype male mice were cultured in KSOM medium for 1 and 3 days to calculate the rates of 2-cell and blastocyst, respectively. Representative images of mouse 2-cell and blastocyst are shown above the bar graph. Experiments were repeated a minimum of three times; the data are presented as mean ± SEM.