Research Paper Volume 13, Issue 1 pp 957—972

Figure 2. The activation of hepatic stellate cells induced Bnip3 expression and its cytoplasmic translocation in vitro. LX-2 cells were treated with 100 μM CoCl₂ or 2 μg/ml LPS for 8 h. (A) Cells were collected at indicated time and cell lysates were subjected to detect Bnip3 with Western blot. Densitometric analysis for Western blot was performed and data were expressed as mean ± SD, *P < 0.05. (B) Immunofluorescence assay was performed to detect Bnip3 (Cy3) in CoCl₂- or LPS-treated LX-2 cells. Culture-activated primary HSCs from mice were cultured up to 2 days, 4 days or 6 days. (C) Cells were collected at indicated time and cell lysates were subjected to detect Bnip3 with Western blot. Densitometric analysis for Western blot was performed and data were expressed as mean ± SD, *P < 0.05, **P < 0.01. (D) Immunofluorescence assay was performed to detect Bnip3 (Cy3) and images were captured by confocal microscope.