Figure 4.Tan IIA inhibits oxLDL-induced LOX-1-mediated ROS production and mitochondrial damage in macrophages. (A) Oxidized LDL induction and Tan IIA inhibition on ROS generation detected using ROS fluorescence detector DCFH-DA (ATP treatment as a positive control). (B, C) Oxidized LDL induction and Tan IIA inhibition on mito-ROS production detected with MitoSOX (Invitrogen) using a flow cytometer in mouse B6 macrophages and LOX-1-KD B6 macrophages, respectively. (D) Mito-ROS production in the B6 macrophages induced by oxLDL at the different doses (0, 10, 25, 50, 100 μg/mL).
(E) Inhibition of Tan IIA on oxLDL-induced mito-ROS production in the B6 macrophages. (F) The oxLDL-induced mitochondria damage and Tan IIA inhibition on it in mouse B6 macrophages and LOX-1-KD macrophages. MitoTracker Deep Red for health mitochondria staining and MitoTracker Green FM for total mitochondria staining. NAC is a ROS inhibitor. (G) The oxLDL-induced mitochondrial membrane potential loss. (H) Tan IIA inhibition on oxLDL-induced mitochondrial membrane potential loss. ATP as a positive control for oxLDL-induced the loss of mitochondrial membrane potential.
