Research Paper Volume 13, Issue 1 pp 389—410

Figure 3. GPR84 proteins are physically associated with DOK3 in microglia. (A) Microglia were pretreated with LPS (1 μM) for 4 or 8 h, and the levels of GPR84 and DOK3 protein were assayed by immunofluorescence analysis. (B) Microglia were pretreated with 6-OAU (1μ M) for 1 or 2 h, and the levels of GPR84 and DOK3 protein were assayed by immunofluorescence analysis. Scale bar, 10 μm. (C) Microglia were pretreated with LPS (1 μM) for 4 h, and the levels of GPR84 in total cell lysates were determined by co-immunoprecipitation and western blotting. (D) Purified recombinant human GST or GST-DOK3 proteins were incubated with microglia for 2 h in reaction buffers. Total cell lysates or reaction products were then used in GST-pulldown assays and western blotting to determine the levels of GPR84.