Figure 6. Effects of PTX treatment on Nrf2 expression in the aged rat brain. (A, B) Nrf2 mRNA levels in the SN and HIPP were calculated using the 2-ΔΔCt method. GAPDH was used as an internal control. (C, D) Representative Western blots of Nrf2 protein levels in the SN and HIPP. (E, F) Nrf2 protein levels in the SN and HIPP were quantified relative to H3 band density. Data are expressed as the mean ± S.D. (n=6 rats/group). *P<0.01.