Research Paper Volume 13, Issue 1 pp 340—350

Figure 1. LINC00052 is downregulated miR-532-3p is upregulated in AKI patient tissues and AKI cell models. (A) Analysis of LINC00052 expression in serum from healthy controls (n = 100) and AKI patients (n = 100). GAPDH served as a loading control. (B) miR-532-3p expression in serum from healthy controls (n = 100) and AKI patients (n = 100). U6 served as a loading control. (C) The expression of IL-1β, IL-6 and TNF-α mRNAs in NRK-52E cells exposed to hypoxia. (D) Apoptosis of NRK-52E cells was measured by flow cytometry. NRK-52E cells were exposed to a hypoxia time-course. (E) Analysis of LINC00052 expression in NRK-52E cells after 0, 3 h, 6 h, 9 h or 12 h of hypoxia. (F) Analysis of miR-532-3p expression in NRK-52E cells after 0, 3 h, 6 h, 9 h and 12 h of hypoxia. Three independent experiments were performed. Error bars represent the mean ± SD of triplicate experiments (at least). *p < 0.05; **p < 0.01.