Research Paper Volume 12, Issue 23 pp 24208—24218

Figure 2. SRT2183 induces autophagy in ovarian cancer cells. (A) OVCAR-3 and A2780 cells were treated with vehicle or 1 μM SRT2183, then observed by transmission electron micrograph (TEM). (B) After incubation with 1 μM SRT2183, the expression of LC3 and p62/ SQSTM1 was detected by western blot. (C) OVCAR-3 and A2780 cells were pre-transfected with GFP-LC3, after 24 h the cells were treated with vehicle or 1 μM SRT2183 for 24 h. The LC3 was observed by confocal microscope. (D) OVCAR-3 and A2780 cells were transfected with GFP- RFP-LC3 for 24 h and pre-treated with chloroquine (CQ) for 6 h, then the cells were treated with vehicle or 1 μM SRT2183 for 24 h. Images were obtained by a confocal microscope. Red dot (GFP−, RFP+): autolysosomes; yellow dot (GFP+, RFP+): autophagosome. N=3 for (A), (B) and (D). N=6 for (C), *** indicates P < 0.001.