Research Paper Volume 12, Issue 24 pp 25189—25206

Figure 6. SETD2-catalyzed H3K36me3 interacts with CXCL1 promoter to regulate CXCL1 transcription. (A) A schematic diagram showing the CXCL1 promoter luciferase reporter vectors with four distinct promoter regions, which then were co-transfected with Lv-SETD2-shRNA1, Lv-SETD2-shRNA2, or Lv-Scramble into H1650 and PC-9 cells and subjected to luciferase activity assays. (B) Western blotting analyses of H3K36me3 levels upon SETD2 overexpression or depletion in H1650 and PC-9 cells. Quantitative results were indicated on the right panel. (C) ChIP-qPCR assays of SETD2 and H3K36me3 in CXCL1 gene. (D) ChIP assay of H3K36me3 in CXCL1 promoter. (E) Western blotting analyses of phosphorylated and total protein level of ERK, JAK2 and STAT3 upon SETD2 overexpression or depletion in H1650 and PC-9 cells. Quantitative results were indicated on the right panel. *P<0.05, **P<0.01. Two-tailed Student’s t test.