Research Paper Volume 12, Issue 24 pp 25189—25206

Figure 3. Deficiency of SETD2 improves cancer cell growth in vitro. (A) Real-time qPCR confirmed the downregulated expression level of SETD2 in lung cancer cells H1650 and PC-9. (B) Western blotting analyses of SETD2 deficiency in H1650 and PC-9 cells. Quantitative results were shown in the right panel. (C) Cell proliferation assays of SETD2 deficiency H1650 and PC-9 cells. (D) Anchorage-independent growth assays of SETD2 deficiency H1650 and PC-9 cells. Quantitative results were indicated in the right panel. (E) EDU staining of SETD2 down-regulated H1650 and PC-9 cells (×200). (F) Cell cycle analysis of SETD2 deficiency H1650 and PC-9 cells. Proportion of cells in G0-G1 phase, S phase and G2-M phase was quantified. (G) Apoptosis analysis of SETD2 down-regulated H1650 and PC-9 cells by Annexin V/PI assay. *P<0.05. Two-tailed Student’s t test.