Figure 2.Validation of gene expression of top dynamic genes in the ConA hepatitis model mice. (A) The expression of two genes with the earliest or latest breakpoint time (Mmp3 at 3 h, and Aadac at 24 h) as determined by Trendy (upper panel) were verified by qRT-PCR analysis (bottom panel) of the liver tissues from the in vivo ConA-treated hepatitis model mice. (B) qRT-PCR results show the mRNA levels of the top 5 dynamic genes (Cd63, Saa3, Slc10a1, Nrxn1, Ugt2a3) in the liver tissues of the ConA-liver injury model mice at 0h, 3h, and 24 h respectively. All data are shown as means ± SEM (n = 5 per group). * p < 0.05; ** p < 0.01; *** p < 0.001.
