Expression of Concern
This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
This article is currently under investigation. We strongly recommend that this article is not cited until the investigation is completed.
Research Paper Volume 12, Issue 23 pp 24009—24022
Extracellular vesicle derived miR-544 downregulates expression of tumor suppressor promyelocytic leukemia zinc finger resulting in increased peritoneal metastasis in gastric cancer
Figure 5. miR-544 is transferred from GS-derived EVs to peritoneal cells. (A) RT-PCR of miR-544 in GC tissues. (B) RT-PCR of miR-544 in EVs isolated from GC patients with and without PM. (n=68 for GC patients without PM, n=65 for GC patients with PM, two-tailed unpaired student’s t-tests) (C) RT-PCR of miR-544 in GC cell lines MGC803, BGC823, MKN45, HGC27, and SGC7901. (D) RT-PCR of miR-544 in EVs isolated from GC and GES-1 cells. (E) PKH67-labeled EVs in HMrSV5 cells (bar represents 10 μm). (F) PLZF expression in HMrSV5 cells co-cultured with MGC-803-and GES-1-derived EVs. (n=3, one way ANOVA for C, D, E and F).